The holders are left needing to better Sweden's result in the final round of games - against South Korea and Mexico respectively - if they are to go through, while the chances of Germany meeting Brazil in the round of 16 boosted by Mexico's earlier win over South Korea.
Mexico built on their opening game victory over Germany by beating South Korea 2-1 in Rostov-on-Don, thanks to a penalty strike from Carlos Vela and a second-half goal from Javier Hernandez.
Mexico will reach the last 16 should Sweden beat Germany in Saturday's final game.
"We can not afford to get caught up in all this, we have to continue our job and stay humble, of course we should enjoy this but tomorrow we start working again".
Marco Reus equalized in the 48th minute, meeting a low cross from Timo Werner, and despite having Jerome Boateng sent off in the 82nd minute, Germany kept going and Kroos earned them a famous win.
Osorio admitted he didn't know exactly how South Korea would be lining up tactically in Rostov, but said he had a plan A and plan B depending on how the Asian side approach the game.
Toni Kroos' last-minute victor for Germany against Sweden later on Saturday means Mexico need a point against Sweden in their final group match to guarantee progress.
"We can not afford to get caught up in all this, we have to continue our job and stay humble, of course we should enjoy this but tomorrow we start working again". "We have a good group of players and human beings".More news: ACLU, Sanders, Meeks Slam Trump's Call to Deport Immigrants Without Cause
After the 1-0 win, Mexico players said that their belief that they could go toe-to-toe with Germany was key to springing the upset.
Carlos Vela put them ahead from the penalty spot in the 26th minute at Rostov Arena, which had been taken over by Mexico's sea of fanatical supporters.
"I think we have a very [united] group of professionals with Andres, our captain, to my right".
Last week, Mexico shocked reigning champions Germany 1-0 in the teams' first match of the tournament.
- South Korea has lost its last four World Cup matches, tied for its worst losing run at the World Cup (it also lost four straight between 1986 and 1990).
Mexico were quick out of the traps in the opening half, pressing forward and passing well around the Korean team.
"We did explain the strong points and weak points of the Mexican team".
South Korea: Cho Hyun-Woo 5, Lee Yong 5, Jang Hyun-Soo 5, Kim Young-Gwon 5, Kim Min-Woo 5, Moon Seon-Min 6, Ju Se-Jong 5, Ki Sung-Yueng 6, Lee Jae-Sung 5, Son Heung-Min 7.