Singer outlined the entire scheme and secretly recorded calls with parents who allegedly paid him thousands of dollars to gain their children admission to elite colleges.
Huffman is scheduled to appear in federal court in Boston on Friday, March 29.
Loughlin, the 54-year-old star of "Full House", and her fashion designer husband Mossimo Giannulli allegedly paid $500,000 to gain their two daughters entry into University of Southern California as coxswains for crew teams - a sport they hadn't participated in before.
Meanwhile, William "Rick" Singer, 58, was charged racketeering conspiracy, money laundering conspiracy, conspiracy to defraud United States and obstruction of justice for his alleged involvement in the scheme. E! She has lately become the queen of the Hallmark Channel with her holiday movies and the series "When Calls the Heart". Felicity Huffman, who starred in ABC's Desperate Housewives, is also being charged.
A judge set bond for Huffman, 56, at $250,000. A college prep school director is charged, as is the director of exam-preparation company.
The announcement comes a day after the nationwide scandal exposed what federal prosecutors describe as a corrupt exchange of wealth, fame and influence for student admissions to the nation's most elite universities.
The elaborate scheme involved bribing the administrators of college entrance tests to allow a child's wrong answers to be corrected and bribing university athletic coaches to attest a child was a gifted athlete even if he or she was anything but.
The list of impacted schools includes Yale, Stanford, the University of Southern California, Wake Forest, Georgetown, UCLA, and more.More news: Germany faces losing USA intelligence if it uses Huawei equipment
Actresses Felicity Huffman and Lori Loughlin are among 50 people arrested by the Federal Bureau of Investigation for allegedly taking part in the "largest college admissions scam every prosecuted by the Department of Justice".
U.S. actresses Felicity Huffman and Lori Loughlin and some CEOs are among dozens of people arrested for a $US25 million ($A35 million) scheme to help wealthy Americans cheat their children's way into elite universities, such as Yale and Stanford.
"For every student admitted through fraud, an honest and genuinely talented student was rejected", Lelling said.
Numerous children were unaware of their parents' activities.
According to court documents, Huffman's daughter saw an increase of 400 points between the first and second time she took the SATs. According to multiple reports, the actress landed at LAX on Tuesday during the court hearing.
Singer ended up cooperating with investigators past year, helping them record incriminating conversations he had with Loughlin, Henriquez and other parents.
Mr Macy has not been charged, but authorities have not said why.
According to the AP news agency, the documents say a co-operating witness met with Huffman and husband Macy at their home in LA.